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KMID : 0363320130340010100
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Journal of Korean Oriental Internal Medicine
2013 Volume.34 No. 1 p.100 ~ p.111
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The impact of Caesalpinia Sappan L. on Oxidative Damage and Inflammatory Relevant Factor in RAW 264.7 Cells and HUVEC
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Kang Seong-Sun
Kim Myung-Sin
Jo Jae-Joon
Choi Seong-An
Yang Eui-Ho
Jeon Sang-Yun
Choi Chang-Won
Hong Seok
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Abstract
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Objectives : This study investigated the impact of Caesalpinia sappan L. on oxidative damage and inflammatory relevant factor in RAW 264.7 cells and human umbilical vein endothelial cells (HUVEC).
Methods : We determined whether fractionated EtOH extracts of Caesalpinia sappan L. (CSL) inhibit free radical generation such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), reactive oxygen species (ROS) and nitric oxide (NO) and pro-inflammatory cytokines in lipopolysaccharide (LPS)-treated RAW 264.7 cells and HUVEC.
Result : 1. DPPH removal capacity was increased by CSL. 2. LPS-induced ROS, and NO inhibitory capacity were increased by CSL. 3. LPS-induced cell death of Raw 264.7 cells was decreased by CSL. 4. The amount of cytokine generation in Raw 264.7 cell was decreased significantly by CSL. 5. The amount of cytokine generation in HUVEC was decreased significantly by CSL.
Conclusions : These results suggest that CSL supplement may attenuate oxidative stress by elevated antioxidative processes, and suppress inflammatory mediator activation.
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KEYWORD
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Caesalpinia sappan L., Raw 264.7 cell, HUVEC, oxidative stress, inflammation, cytokine
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